First Report of <i>Colletotrichum siamense</i> Causing Anthracnose on White Frangipani (<i>Plumeria alba</i>) in Malaysia

HomePlant DiseaseVol. 105, No. 9First Report of Colletotrichum siamense Causing Anthracnose on White Frangipani (Plumeria alba) in Malaysia PreviousNext DISEASE NOTE OPENOpen Access licenseFirst MalaysiaS. I. Ismail, N. L. Mohmad Zaiwawi, S. Abdullah, Jamian, and SaadS. Ismail†Corresponding author: Ismail; E-mail Address: [email protected]https://orcid.org/0000-0003-1904-4890Department Plant Protection, Faculty Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaSearch for more papers by this author, ZaiwawiDepartment AbdullahDepartment JamianDepartment Saadhttps://orcid.org/0000-0003-1937-9500Department authorAffiliationsAuthors Affiliations Ismail † Zaiwawi Abdullah Jamian Saad Department Published Online:27 Sep 2021https://doi.org/10.1094/PDIS-12-20-2614-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articlePlumeria alba is a flowering plant the family Apocynaceae widely cultivated as cosmopolitan ornamental plant. In January 2020, anthracnose lesions were observed leaves P. planted Agricultural Farm, Selangor state, Malaysia. The disease mainly affected leaves, with symptoms occurring approximately 60% incidence. Ten symptomatic sampled from three different trees farm. Symptoms initiated small circular necrotic spots that rapidly enlarged into black pale brown borders. Diseased tissues (5 × 5 mm) surface sterilized 70% ethanol 1 min, rinsed times sterile distilled water, dried filter papers, plated potato dextrose agar (PDA), incubated at 25°C 12-h photoperiod. A total seven single-spore isolates similar colony morphologies obtained tissue samples. After 7 days, colonies raised entire margin showed white-to-gray aerial mycelium, orange conidial masses center, appeared dark center reverse view. conidia one-celled, hyaline, smooth-walled, cylindrical narrowing averaged 13 15 3 4 ?m (n = 40) size. Morphological characteristics those detailed taxonomic description sp. (Prihastuti et al. 2009). For molecular identification, genomic DNA two representative isolates, PL3 PL4, was extracted fresh mycelium using DNeasy Mini Kit (Qiagen, U.S.A.). internal transcribed spacer (ITS) region, actin (ACT), calmodulin (CAL) genes amplified ITS5/ITS4 (White 1990), ACT-512F/783R (Carbone Kohn 1999), CL1C/CL2C primer sets (Weir 2012). BLAST nucleotide search GenBank ITS sequences 100% identity ex-type culture ICMP 18578 (GenBank accession no. JX010171). ACT CAL C. isolate BPD-I2 nos. FJ907423 FJ917505). deposited (ITS, MW335128, MT912574; ACT, MW341257, MW341256; CAL, MW341255, MT919260). Based these morphological characteristics, fungus identified siamense. Pathogenicity PL4 verified four healthy detached alba. twice water before inoculation. (three inoculation sites/leaf) wounded puncturing needle through leaf cuticle inoculated wound site 10 ?l suspension (1 106 conidia/ml) 7-day-old PDA. Four used control only water. Inoculated kept humid chambers 2 weeks 98% relative humidity fluorescent light/dark period. experiment repeated times. all after whereas controls no symptoms. Fungal diseased same confirming Koch’s postulates. has been reported causing rose (Rosa chinensis) China (Feng 2019), Coffea arabica Thailand 2009), mango Vietnam (Li 2020). To our knowledge, first report Accurate identification pathogen provides foundation controlling alba.The author(s) declare conflict interest.References:Carbone, I., Kohn, M. 1999. Mycologia 91:553. https://doi.org/10.1080/00275514.1999.12061051 Crossref, ISI, Google ScholarFeng, F. S., 2019. Dis. 103:1422. https://doi.org/10.1094/PDIS-11-18-1966-PDN Link, ScholarLi, Q., 2020. 104:1558. https://doi.org/10.1094/PDIS-09-19-1830-PDN ScholarPrihastuti, H., 2009. Divers. 39:89. ScholarWeir, B. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 ScholarWhite, T. J., 1990. Page 315 in: PCR Protocols: Guide Methods Applications. Academic Press, San Diego, CA. ScholarThe interest.Funding: This study funded Grant (UPM) (grant 9675300).DetailsFiguresLiterature CitedRelated Vol. 9 September 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionKadsura coccinea showing spot caused Botryosphaeria dothidea (D. Su al.). Photo credit: D. Su. Leaf Ramularia coleosporii Perilla (M. Aktaruzzaman Kim. Injection maize classification standard stalk rot (W. Y. Jiang G. Li. Metrics Article History Issue Date: 8 Dec 2021Published: 27 2021First Look: Apr 2021Accepted: 14 2021 Page: 2714 Information© American Phytopathological SocietyFundingPutra (UPM)Grant/Award Number: 9675300KeywordsColletotrichum siamensePlumeria L.anthracnosewhite frangipaniThe interest.PDF downloadCited byMorphology, phylogeny, pathogenicity associated blight pod Theobroma cacao Malaysia1 March 2023 | Tropical Pathology, 72Colletotrichum species complexes: geographic distribution, host range conservation status29 Diversity, 110,